Journal: PLoS ONE

Article Title: The Curcumin Analog EF24 Targets NF-κB and miRNA-21, and Has Potent Anticancer Activity In Vitro and In Vivo

doi: 10.1371/journal.pone.0071130

Figure Lengend Snippet: DU145 cells plated on 8-well chamber slides were treated with EF24 (5 µM) or vehicle (control) for 24 hr prior to stimulation with human IFNα (1000 IU/ml) for 30 min. Cells were fixed with 4% paraformaldehyde and methanol, and permeabilized with 1% Triton X100. After blocking with 5% goat serum, slides were stained for p65 and mounted using Vectashield medium with DAPI, and images were captured on a Zeiss LSM700 laser scanning confocal microscope.

Article Snippet: After incubation with goat anti-rabbit (or rabbit anti-mouse) Alexa Fluor 488 secondary antibody at 25°C for 90 min, DNA was counterstained with Vectashield mounting media with DAPI (Vectra Laboratories).

Techniques: Control, Blocking Assay, Staining, Microscopy